Due to the way the rolling window is done, not the entire DAPI region is covered equally. Central regions are covered more frequently than edge regions.
This could potentially be fixed by replacing the sliding window method with a window around each spot and counting how many other spots are inside. Some code for this is already present in the alt_windows branch, but has not been implemented to run smoothly, e.g. with centrosome region exclusion.
Due to the way the rolling window is done, not the entire DAPI region is covered equally. Central regions are covered more frequently than edge regions.
This could potentially be fixed by replacing the sliding window method with a window around each spot and counting how many other spots are inside. Some code for this is already present in the alt_windows branch, but has not been implemented to run smoothly, e.g. with centrosome region exclusion.