bio ass1.ipynb

{
 "cells": [
  {
   "cell_type": "code",
   "execution_count": 1,
   "source": [
    "Pu = (\"A\",\"G\")\r\n",
    "Py = (\"T\",\"C\")\r\n",
    "\r\n",
    "def Comp(seq):\r\n",
    "   comp=\"\"\r\n",
    "   for i in range(len(seq)):\r\n",
    "       if(seq[i] in Pu):\r\n",
    "           if(seq[i]==Pu[0]):\r\n",
    "                comp+=Py[0]\r\n",
    "           else:\r\n",
    "                comp+=Py[1]\r\n",
    "       elif(seq[i] in Py):\r\n",
    "           if (seq[i] == Py[0]):\r\n",
    "                comp+=Pu[0]\r\n",
    "           else:\r\n",
    "                comp+=Pu[1]\r\n",
    "       else:\r\n",
    "           comp+=\"\"\r\n",
    "   return comp\r\n",
    "def Frames(seq):\r\n",
    "    Frames=[]\r\n",
    "    for i in range(3):\r\n",
    "        seqt=seq[i:]\r\n",
    "        Frames.insert(i,seqt[:(len(seqt)-(len(seqt)%3))])\r\n",
    "    seq=Comp(seq)\r\n",
    "    seq=seq[::-1]\r\n",
    "    for i in range(3):\r\n",
    "        seqt = seq[i:]\r\n",
    "        Frames.insert(i+3, seqt[:(len(seqt) - (len(seqt) % 3))])\r\n",
    "    return Frames\r\n",
    "def Translation(seq):\r\n",
    "    def Process(seq):\r\n",
    "        table = {\r\n",
    "            'ATA': 'I', 'ATC': 'I', 'ATT': 'I', 'ATG': 'M',\r\n",
    "            'ACA': 'T', 'ACC': 'T', 'ACG': 'T', 'ACT': 'T',\r\n",
    "            'AAC': 'N', 'AAT': 'N', 'AAA': 'K', 'AAG': 'K',\r\n",
    "            'AGC': 'S', 'AGT': 'S', 'AGA': 'R', 'AGG': 'R',\r\n",
    "            'CTA': 'L', 'CTC': 'L', 'CTG': 'L', 'CTT': 'L',\r\n",
    "            'CCA': 'P', 'CCC': 'P', 'CCG': 'P', 'CCT': 'P',\r\n",
    "            'CAC': 'H', 'CAT': 'H', 'CAA': 'Q', 'CAG': 'Q',\r\n",
    "            'CGA': 'R', 'CGC': 'R', 'CGG': 'R', 'CGT': 'R',\r\n",
    "            'GTA': 'V', 'GTC': 'V', 'GTG': 'V', 'GTT': 'V',\r\n",
    "            'GCA': 'A', 'GCC': 'A', 'GCG': 'A', 'GCT': 'A',\r\n",
    "            'GAC': 'D', 'GAT': 'D', 'GAA': 'E', 'GAG': 'E',\r\n",
    "            'GGA': 'G', 'GGC': 'G', 'GGG': 'G', 'GGT': 'G',\r\n",
    "            'TCA': 'S', 'TCC': 'S', 'TCG': 'S', 'TCT': 'S',\r\n",
    "            'TTC': 'F', 'TTT': 'F', 'TTA': 'L', 'TTG': 'L',\r\n",
    "            'TAC': 'Y', 'TAT': 'Y', 'TAA': '', 'TAG': '',\r\n",
    "            'TGC': 'C', 'TGT': 'C', 'TGA': '*', 'TGG': 'W',\r\n",
    "        }\r\n",
    "        protein = \"\"\r\n",
    "        for i in range(0, len(seq), 3):\r\n",
    "            codon = seq[i:i + 3]\r\n",
    "            protein += table[codon] + \" \"\r\n",
    "        return protein\r\n",
    "    aa = {\r\n",
    "        'A': 'Ala', 'C': 'Cys', 'D': 'Asp', 'E': 'Glu',\r\n",
    "        'F': 'Phe', 'G': 'Gly', 'H': 'His', 'I': 'Ile',\r\n",
    "        'K': 'Lys', 'L': 'Lue', 'M': 'Met', 'N': 'Asn',\r\n",
    "        'P': 'Pro', 'Q': 'Gln', 'R': 'Arg', 'S': 'Ser',\r\n",
    "        'T': 'Thr', 'V': 'Val', 'W': 'Trp', 'Y': 'Tyr'\r\n",
    "    }\r\n",
    "    F = Frames(seq)\r\n",
    "    Protein = []\r\n",
    "    tran = []\r\n",
    "\r\n",
    "    for i in F:\r\n",
    "        Protein.append(Process(i))\r\n",
    "    for i in Protein:\r\n",
    "        s = \"\"\r\n",
    "        for j in range(len(i)):\r\n",
    "            if (i[j] == '*'):\r\n",
    "                s += '* '\r\n",
    "            elif (i[j] != \" \"):\r\n",
    "                s += aa[i[j]] + \" \"\r\n",
    "        tran.append(s)\r\n",
    "    return Protein,tran\r\n",
    "def ORF(seq):\r\n",
    "    def ORFs(seq):\r\n",
    "\r\n",
    "        Start = \"ATG\"\r\n",
    "        Stop = [\"TAA\", \"TAG\", \"TGA\"]\r\n",
    "        bool = j = 0\r\n",
    "        orf = []\r\n",
    "\r\n",
    "        while (j < len(seq)):\r\n",
    "            s1 = s2 = None\r\n",
    "            bool = 0\r\n",
    "            for i in range(j, len(seq), 3):\r\n",
    "                codon = seq[i:i + 3]\r\n",
    "                if (codon == Start and bool == 0):\r\n",
    "                    bool = 1\r\n",
    "                    s1 = i\r\n",
    "\r\n",
    "                if (codon in Stop and bool == 1):\r\n",
    "                    s2 = i + 3\r\n",
    "\r\n",
    "                    break\r\n",
    "\r\n",
    "            if (s1 != None):\r\n",
    "                if (s2 == None):\r\n",
    "\r\n",
    "                    s2 = len(seq) - (len(seq[s1:]) % 3)\r\n",
    "                    orf.append(seq[s1:s2])\r\n",
    "                    j = len(seq)\r\n",
    "                else:\r\n",
    "                    j = s1 + 3\r\n",
    "                    orf.append(seq[s1:s2])\r\n",
    "            else:\r\n",
    "                orf.append(\"\")\r\n",
    "                j = len(seq)\r\n",
    "\r\n",
    "        return orf\r\n",
    "    F = Frames(seq)\r\n",
    "    orf = {'Frame 1': ORFs(F[0]), 'Frame 2': ORFs(F[1]),\r\n",
    "           'Frame 3': ORFs(F[2]), 'Frame 4': ORFs(F[3]),\r\n",
    "           'Frame 5': ORFs(F[4]), 'Frame 6': ORFs(F[5]),\r\n",
    "           }\r\n",
    "\r\n",
    "    return orf\r\n",
    "def findLongORF(seq):\r\n",
    "    orf=ORF(seq)\r\n",
    "    New=[]\r\n",
    "    New.extend(orf[\"Frame 1\"])\r\n",
    "    New.extend(orf[\"Frame 2\"])\r\n",
    "    New.extend(orf[\"Frame 3\"])\r\n",
    "    New.extend(orf[\"Frame 4\"])\r\n",
    "    New.extend(orf[\"Frame 5\"])\r\n",
    "    New.extend(orf[\"Frame 6\"])\r\n",
    "    gt=New[0]\r\n",
    "    for i in range(1,len(New)):\r\n",
    "        if(len(gt)<len(New[i])):\r\n",
    "            gt=New[i]\r\n",
    "\r\n",
    "    for i in orf:\r\n",
    "        if(gt in orf[i]):\r\n",
    "            index=\"Number \"+str(orf[i].index(gt)+1)+\" Translational ORF in \"+i\r\n",
    "    return index,gt\r\n",
    "def findRatio(seq):\r\n",
    "    A=seq.count(\"A\")\r\n",
    "    T=seq.count(\"T\")\r\n",
    "    netP1=2*(A+T)\r\n",
    "    G = seq.count(\"G\")\r\n",
    "    C = seq.count(\"C\")\r\n",
    "    netP2 = 2 * (G + C)\r\n",
    "    ratio=str(netP1)+\" : \"+str(netP2)\r\n",
    "    return ratio\r\n",
    "\r\n",
    "seq=\"TTTAGGACTTGACAGTTTCGGTCCGGAATTCCCGGGATCTACT\"             \\\r\n",
    "    \"ACAAACAAATTACATTCAATATGCTTTTCTCAAGAGACTTTATT\"            \\\r\n",
    "    \"ATGCTTTTGGCATTCGCCGCCGTTGGCGCTGTCGCTGCACCTGTTG\"          \\\r\n",
    "    \"CAGAGCCTCAACCAGAAGCCAAAGCTGATGCTTCTCCAGCGGACTAT\"         \\\r\n",
    "    \"GGCGACTACGGTGACTATGGCGATTACGGAAACTATGGAACATACGAGAA\"      \\\r\n",
    "    \"ATACCCATCCTCTTCCTCCGTCACACCTACACCAACACCAACACCAACTGGAT\"   \\\r\n",
    "    \"ATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAG\"        \\\r\n",
    "    \"AAGCCGCCCCAGAGCCTGCTCCAGTAGCAAAGCGTGATGATTATGGAAACTAT\"   \\\r\n",
    "    \"GGAGACTATGGTGACTACGGAAACTACGGCAACTACGAGAACTACCCTTCCG\"    \\\r\n",
    "    \"CTACCTCTTCTGCTACCTCTTCTGCCACCCCTTCAAGCTCCTCGTCAAGCTCT\"   \\\r\n",
    "    \"TCATCAGCTACACCAACACCAACTCCAACCGGTTACGGTTCATACGCAAGCTACGG\"\\\r\n",
    "    \"AGCATACAAGAACAAGAAAGCACGTGCTCCAGAAGCCAACCCAGAAGACTATGGAA\"\\\r\n",
    "    \"ACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTG\"        \\\r\n",
    "    \"CCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTAC\"   \\\r\n",
    "    \"CCAACGGCTACACAACTACGGCTCCTAAGTACTACGACACTACAGCGCGAAGC\"   \\\r\n",
    "    \"GATACGTTCAGCTTAGCGATGACTATTGACAGTTTATTGTATACACACTCTTAGAC\"\\\r\n",
    "    \"GATGAATCGCCTTTAATATATGATATAATGATCAATGTTGACTGCCTCGCACTGAT\"\\\r\n",
    "    \"TGGAGAATGGATAAAAG\"\r\n",
    "\r\n",
    "f=Frames(seq)\r\n",
    "print(\"Frame 1\")\r\n",
    "print(f[0])\r\n",
    "print(\"Frame 2\")\r\n",
    "print(f[1])\r\n",
    "print(\"Frame 3\")\r\n",
    "print(f[2])\r\n",
    "print(\"Frame 4\")\r\n",
    "print(f[3])\r\n",
    "print(\"Frame 5\")\r\n",
    "print(f[4])\r\n",
    "print(\"Frame 6\")\r\n",
    "print(f[5])\r\n",
    "print()\r\n",
    "\r\n",
    "Result = Translation(seq)\r\n",
    "print(Result[0][0])\r\n",
    "print(Result[1][0])\r\n",
    "print()\r\n",
    "print(Result[0][1])\r\n",
    "print(Result[1][1])\r\n",
    "print()\r\n",
    "print(Result[0][2])\r\n",
    "print(Result[1][2])\r\n",
    "print()\r\n",
    "print(Result[0][3])\r\n",
    "print(Result[1][3])\r\n",
    "print()\r\n",
    "print(Result[0][4])\r\n",
    "print(Result[1][4])\r\n",
    "print()\r\n",
    "print(Result[0][5])\r\n",
    "print(Result[1][5])\r\n",
    "print()\r\n",
    "print(\"Frame 1\")\r\n",
    "print(ORF(seq)[\"Frame 1\"])\r\n",
    "print(\"Frame 2\")\r\n",
    "print(ORF(seq)[\"Frame 2\"])\r\n",
    "print(\"Frame 3\")\r\n",
    "print(ORF(seq)[\"Frame 3\"])\r\n",
    "print(\"Frame 4\")\r\n",
    "print(ORF(seq)[\"Frame 4\"])\r\n",
    "print(\"Frame 5\")\r\n",
    "print(ORF(seq)[\"Frame 5\"])\r\n",
    "print(\"Frame 6\")\r\n",
    "print(ORF(seq)[\"Frame 6\"])\r\n",
    "print()\r\n",
    "print(findLongORF(seq))\r\n",
    "print(findRatio(seq))"
   ],
   "outputs": [
    {
     "output_type": "stream",
     "name": "stdout",
     "text": [
      "Frame 1\n",
      "TTTAGGACTTGACAGTTTCGGTCCGGAATTCCCGGGATCTACTACAAACAAATTACATTCAATATGCTTTTCTCAAGAGACTTTATTATGCTTTTGGCATTCGCCGCCGTTGGCGCTGTCGCTGCACCTGTTGCAGAGCCTCAACCAGAAGCCAAAGCTGATGCTTCTCCAGCGGACTATGGCGACTACGGTGACTATGGCGATTACGGAAACTATGGAACATACGAGAAATACCCATCCTCTTCCTCCGTCACACCTACACCAACACCAACACCAACTGGATATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAGCAAAGCGTGATGATTATGGAAACTATGGAGACTATGGTGACTACGGAAACTACGGCAACTACGAGAACTACCCTTCCGCTACCTCTTCTGCTACCTCTTCTGCCACCCCTTCAAGCTCCTCGTCAAGCTCTTCATCAGCTACACCAACACCAACTCCAACCGGTTACGGTTCATACGCAAGCTACGGAGCATACAAGAACAAGAAAGCACGTGCTCCAGAAGCCAACCCAGAAGACTATGGAAACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTGCCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTACCCAACGGCTACACAACTACGGCTCCTAAGTACTACGACACTACAGCGCGAAGCGATACGTTCAGCTTAGCGATGACTATTGACAGTTTATTGTATACACACTCTTAGACGATGAATCGCCTTTAATATATGATATAATGATCAATGTTGACTGCCTCGCACTGATTGGAGAATGGATAAA\n",
      "Frame 2\n",
      "TTAGGACTTGACAGTTTCGGTCCGGAATTCCCGGGATCTACTACAAACAAATTACATTCAATATGCTTTTCTCAAGAGACTTTATTATGCTTTTGGCATTCGCCGCCGTTGGCGCTGTCGCTGCACCTGTTGCAGAGCCTCAACCAGAAGCCAAAGCTGATGCTTCTCCAGCGGACTATGGCGACTACGGTGACTATGGCGATTACGGAAACTATGGAACATACGAGAAATACCCATCCTCTTCCTCCGTCACACCTACACCAACACCAACACCAACTGGATATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAGCAAAGCGTGATGATTATGGAAACTATGGAGACTATGGTGACTACGGAAACTACGGCAACTACGAGAACTACCCTTCCGCTACCTCTTCTGCTACCTCTTCTGCCACCCCTTCAAGCTCCTCGTCAAGCTCTTCATCAGCTACACCAACACCAACTCCAACCGGTTACGGTTCATACGCAAGCTACGGAGCATACAAGAACAAGAAAGCACGTGCTCCAGAAGCCAACCCAGAAGACTATGGAAACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTGCCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTACCCAACGGCTACACAACTACGGCTCCTAAGTACTACGACACTACAGCGCGAAGCGATACGTTCAGCTTAGCGATGACTATTGACAGTTTATTGTATACACACTCTTAGACGATGAATCGCCTTTAATATATGATATAATGATCAATGTTGACTGCCTCGCACTGATTGGAGAATGGATAAAA\n",
      "Frame 3\n",
      "TAGGACTTGACAGTTTCGGTCCGGAATTCCCGGGATCTACTACAAACAAATTACATTCAATATGCTTTTCTCAAGAGACTTTATTATGCTTTTGGCATTCGCCGCCGTTGGCGCTGTCGCTGCACCTGTTGCAGAGCCTCAACCAGAAGCCAAAGCTGATGCTTCTCCAGCGGACTATGGCGACTACGGTGACTATGGCGATTACGGAAACTATGGAACATACGAGAAATACCCATCCTCTTCCTCCGTCACACCTACACCAACACCAACACCAACTGGATATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAGCAAAGCGTGATGATTATGGAAACTATGGAGACTATGGTGACTACGGAAACTACGGCAACTACGAGAACTACCCTTCCGCTACCTCTTCTGCTACCTCTTCTGCCACCCCTTCAAGCTCCTCGTCAAGCTCTTCATCAGCTACACCAACACCAACTCCAACCGGTTACGGTTCATACGCAAGCTACGGAGCATACAAGAACAAGAAAGCACGTGCTCCAGAAGCCAACCCAGAAGACTATGGAAACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTGCCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTACCCAACGGCTACACAACTACGGCTCCTAAGTACTACGACACTACAGCGCGAAGCGATACGTTCAGCTTAGCGATGACTATTGACAGTTTATTGTATACACACTCTTAGACGATGAATCGCCTTTAATATATGATATAATGATCAATGTTGACTGCCTCGCACTGATTGGAGAATGGATAAAAG\n",
      "Frame 4\n",
      "CTTTTATCCATTCTCCAATCAGTGCGAGGCAGTCAACATTGATCATTATATCATATATTAAAGGCGATTCATCGTCTAAGAGTGTGTATACAATAAACTGTCAATAGTCATCGCTAAGCTGAACGTATCGCTTCGCGCTGTAGTGTCGTAGTACTTAGGAGCCGTAGTTGTGTAGCCGTTGGGTAGGGAGTGGGAGTTGGGGTGACGGTGGATGTGGATGTGGAGGAGGTGGAGGCAGGGGTGTAGTTCTCGTAGTTGCCGTAATCACCGTAGTCACCGTAGTTTCCATAGTCTTCTGGGTTGGCTTCTGGAGCACGTGCTTTCTTGTTCTTGTATGCTCCGTAGCTTGCGTATGAACCGTAACCGGTTGGAGTTGGTGTTGGTGTAGCTGATGAAGAGCTTGACGAGGAGCTTGAAGGGGTGGCAGAAGAGGTAGCAGAAGAGGTAGCGGAAGGGTAGTTCTCGTAGTTGCCGTAGTTTCCGTAGTCACCATAGTCTCCATAGTTTCCATAATCATCACGCTTTGCTACTGGAGCAGGCTCTGGGGCGGCTTCTCTAACTTTCTTGTTCTTGTAGGTACCGTAGCTGGCATAGCTACCATATCCAGTTGGTGTTGGTGTTGGTGTAGGTGTGACGGAGGAAGAGGATGGGTATTTCTCGTATGTTCCATAGTTTCCGTAATCGCCATAGTCACCGTAGTCGCCATAGTCCGCTGGAGAAGCATCAGCTTTGGCTTCTGGTTGAGGCTCTGCAACAGGTGCAGCGACAGCGCCAACGGCGGCGAATGCCAAAAGCATAATAAAGTCTCTTGAGAAAAGCATATTGAATGTAATTTGTTTGTAGTAGATCCCGGGAATTCCGGACCGAAACTGTCAAGTCCTA\n",
      "Frame 5\n",
      "TTTTATCCATTCTCCAATCAGTGCGAGGCAGTCAACATTGATCATTATATCATATATTAAAGGCGATTCATCGTCTAAGAGTGTGTATACAATAAACTGTCAATAGTCATCGCTAAGCTGAACGTATCGCTTCGCGCTGTAGTGTCGTAGTACTTAGGAGCCGTAGTTGTGTAGCCGTTGGGTAGGGAGTGGGAGTTGGGGTGACGGTGGATGTGGATGTGGAGGAGGTGGAGGCAGGGGTGTAGTTCTCGTAGTTGCCGTAATCACCGTAGTCACCGTAGTTTCCATAGTCTTCTGGGTTGGCTTCTGGAGCACGTGCTTTCTTGTTCTTGTATGCTCCGTAGCTTGCGTATGAACCGTAACCGGTTGGAGTTGGTGTTGGTGTAGCTGATGAAGAGCTTGACGAGGAGCTTGAAGGGGTGGCAGAAGAGGTAGCAGAAGAGGTAGCGGAAGGGTAGTTCTCGTAGTTGCCGTAGTTTCCGTAGTCACCATAGTCTCCATAGTTTCCATAATCATCACGCTTTGCTACTGGAGCAGGCTCTGGGGCGGCTTCTCTAACTTTCTTGTTCTTGTAGGTACCGTAGCTGGCATAGCTACCATATCCAGTTGGTGTTGGTGTTGGTGTAGGTGTGACGGAGGAAGAGGATGGGTATTTCTCGTATGTTCCATAGTTTCCGTAATCGCCATAGTCACCGTAGTCGCCATAGTCCGCTGGAGAAGCATCAGCTTTGGCTTCTGGTTGAGGCTCTGCAACAGGTGCAGCGACAGCGCCAACGGCGGCGAATGCCAAAAGCATAATAAAGTCTCTTGAGAAAAGCATATTGAATGTAATTTGTTTGTAGTAGATCCCGGGAATTCCGGACCGAAACTGTCAAGTCCTAA\n",
      "Frame 6\n",
      "TTTATCCATTCTCCAATCAGTGCGAGGCAGTCAACATTGATCATTATATCATATATTAAAGGCGATTCATCGTCTAAGAGTGTGTATACAATAAACTGTCAATAGTCATCGCTAAGCTGAACGTATCGCTTCGCGCTGTAGTGTCGTAGTACTTAGGAGCCGTAGTTGTGTAGCCGTTGGGTAGGGAGTGGGAGTTGGGGTGACGGTGGATGTGGATGTGGAGGAGGTGGAGGCAGGGGTGTAGTTCTCGTAGTTGCCGTAATCACCGTAGTCACCGTAGTTTCCATAGTCTTCTGGGTTGGCTTCTGGAGCACGTGCTTTCTTGTTCTTGTATGCTCCGTAGCTTGCGTATGAACCGTAACCGGTTGGAGTTGGTGTTGGTGTAGCTGATGAAGAGCTTGACGAGGAGCTTGAAGGGGTGGCAGAAGAGGTAGCAGAAGAGGTAGCGGAAGGGTAGTTCTCGTAGTTGCCGTAGTTTCCGTAGTCACCATAGTCTCCATAGTTTCCATAATCATCACGCTTTGCTACTGGAGCAGGCTCTGGGGCGGCTTCTCTAACTTTCTTGTTCTTGTAGGTACCGTAGCTGGCATAGCTACCATATCCAGTTGGTGTTGGTGTTGGTGTAGGTGTGACGGAGGAAGAGGATGGGTATTTCTCGTATGTTCCATAGTTTCCGTAATCGCCATAGTCACCGTAGTCGCCATAGTCCGCTGGAGAAGCATCAGCTTTGGCTTCTGGTTGAGGCTCTGCAACAGGTGCAGCGACAGCGCCAACGGCGGCGAATGCCAAAAGCATAATAAAGTCTCTTGAGAAAAGCATATTGAATGTAATTTGTTTGTAGTAGATCCCGGGAATTCCGGACCGAAACTGTCAAGTCCTAAA\n",
      "\n",
      "F R T * Q F R S G I P G I Y Y K Q I T F N M L F S R D F I M L L A F A A V G A V A A P V A E P Q P E A K A D A S P A D Y G D Y G D Y G D Y G N Y G T Y E K Y P S S S S V T P T P T P T P T G Y G S Y A S Y G T Y K N K K V R E A A P E P A P V A K R D D Y G N Y G D Y G D Y G N Y G N Y E N Y P S A T S S A T S S A T P S S S S S S S S S A T P T P T P T G Y G S Y A S Y G A Y K N K K A R A P E A N P E D Y G N Y G D Y G D Y G N Y E N Y T P A S T S S T S T S T V T P T P T P Y P T A T Q L R L L S T T T L Q R E A I R S A  R * L L T V Y C I H T L R R * I A F N I * Y N D Q C * L P R T D W R M D K \n",
      "Phe Arg Thr * Gln Phe Arg Ser Gly Ile Pro Gly Ile Tyr Tyr Lys Gln Ile Thr Phe Asn Met Lue Phe Ser Arg Asp Phe Ile Met Lue Lue Ala Phe Ala Ala Val Gly Ala Val Ala Ala Pro Val Ala Glu Pro Gln Pro Glu Ala Lys Ala Asp Ala Ser Pro Ala Asp Tyr Gly Asp Tyr Gly Asp Tyr Gly Asp Tyr Gly Asn Tyr Gly Thr Tyr Glu Lys Tyr Pro Ser Ser Ser Ser Val Thr Pro Thr Pro Thr Pro Thr Pro Thr Gly Tyr Gly Ser Tyr Ala Ser Tyr Gly Thr Tyr Lys Asn Lys Lys Val Arg Glu Ala Ala Pro Glu Pro Ala Pro Val Ala Lys Arg Asp Asp Tyr Gly Asn Tyr Gly Asp Tyr Gly Asp Tyr Gly Asn Tyr Gly Asn Tyr Glu Asn Tyr Pro Ser Ala Thr Ser Ser Ala Thr Ser Ser Ala Thr Pro Ser Ser Ser Ser Ser Ser Ser Ser Ser Ala Thr Pro Thr Pro Thr Pro Thr Gly Tyr Gly Ser Tyr Ala Ser Tyr Gly Ala Tyr Lys Asn Lys Lys Ala Arg Ala Pro Glu Ala Asn Pro Glu Asp Tyr Gly Asn Tyr Gly Asp Tyr Gly Asp Tyr Gly Asn Tyr Glu Asn Tyr Thr Pro Ala Ser Thr Ser Ser Thr Ser Thr Ser Thr Val Thr Pro Thr Pro Thr Pro Tyr Pro Thr Ala Thr Gln Lue Arg Lue Lue Ser Thr Thr Thr Lue Gln Arg Glu Ala Ile Arg Ser Ala Arg * Lue Lue Thr Val Tyr Cys Ile His Thr Lue Arg Arg * Ile Ala Phe Asn Ile * Tyr Asn Asp Gln Cys * Lue Pro Arg Thr Asp Trp Arg Met Asp Lys \n",
      "\n",
      "L G L D S F G P E F P G S T T N K L H S I C F S Q E T L L C F W H S P P L A L S L H L L Q S L N Q K P K L M L L Q R T M A T T V T M A I T E T M E H T R N T H P L P P S H L H Q H Q H Q L D M V A M P A T V P T R T R K L E K P P Q S L L Q  Q S V M I M E T M E T M V T T E T T A T T R T T L P L P L L L P L L P P L Q A P R Q A L H Q L H Q H Q L Q P V T V H T Q A T E H T R T R K H V L Q K P T Q K T M E T T V T T V I T A T T R T T P L P P P P P H P H P P S P Q L P L P T Q R L H N Y G S  V L R H Y S A K R Y V Q L S D D Y * Q F I V Y T L L D D E S P L I Y D I M I N V D C L A L I G E W I K \n",
      "Lue Gly Lue Asp Ser Phe Gly Pro Glu Phe Pro Gly Ser Thr Thr Asn Lys Lue His Ser Ile Cys Phe Ser Gln Glu Thr Lue Lue Cys Phe Trp His Ser Pro Pro Lue Ala Lue Ser Lue His Lue Lue Gln Ser Lue Asn Gln Lys Pro Lys Lue Met Lue Lue Gln Arg Thr Met Ala Thr Thr Val Thr Met Ala Ile Thr Glu Thr Met Glu His Thr Arg Asn Thr His Pro Lue Pro Pro Ser His Lue His Gln His Gln His Gln Lue Asp Met Val Ala Met Pro Ala Thr Val Pro Thr Arg Thr Arg Lys Lue Glu Lys Pro Pro Gln Ser Lue Lue Gln Gln Ser Val Met Ile Met Glu Thr Met Glu Thr Met Val Thr Thr Glu Thr Thr Ala Thr Thr Arg Thr Thr Lue Pro Lue Pro Lue Lue Lue Pro Lue Lue Pro Pro Lue Gln Ala Pro Arg Gln Ala Lue His Gln Lue His Gln His Gln Lue Gln Pro Val Thr Val His Thr Gln Ala Thr Glu His Thr Arg Thr Arg Lys His Val Lue Gln Lys Pro Thr Gln Lys Thr Met Glu Thr Thr Val Thr Thr Val Ile Thr Ala Thr Thr Arg Thr Thr Pro Lue Pro Pro Pro Pro Pro His Pro His Pro Pro Ser Pro Gln Lue Pro Lue Pro Thr Gln Arg Lue His Asn Tyr Gly Ser Val Lue Arg His Tyr Ser Ala Lys Arg Tyr Val Gln Lue Ser Asp Asp Tyr * Gln Phe Ile Val Tyr Thr Lue Lue Asp Asp Glu Ser Pro Lue Ile Tyr Asp Ile Met Ile Asn Val Asp Cys Lue Ala Lue Ile Gly Glu Trp Ile Lys \n",
      "\n",
      " D L T V S V R N S R D L L Q T N Y I Q Y A F L K R L Y Y A F G I R R R W R C R C T C C R A S T R S Q S * C F S S G L W R L R * L W R L R K L W N I R E I P I L F L R H T Y T N T N T N W I W  L C Q L R Y L Q E Q E S  R S R P R A C S S S K A * * L W K L W R L W * L R K L R Q L R E L P F R Y L F C Y L F C H P F K L L V K L F I S Y T N T N S N R L R F I R K L R S I Q E Q E S T C S R S Q P R R L W K L R * L R * L R Q L R E L H P C L H L L H I H I H R H P N S H S L P N G Y T T T A P K Y Y D T T A R S D T F S L A M T I D S L L Y T H S  T M N R L  Y M I  * S M L T A S H * L E N G  K \n",
      "Asp Lue Thr Val Ser Val Arg Asn Ser Arg Asp Lue Lue Gln Thr Asn Tyr Ile Gln Tyr Ala Phe Lue Lys Arg Lue Tyr Tyr Ala Phe Gly Ile Arg Arg Arg Trp Arg Cys Arg Cys Thr Cys Cys Arg Ala Ser Thr Arg Ser Gln Ser * Cys Phe Ser Ser Gly Lue Trp Arg Lue Arg * Lue Trp Arg Lue Arg Lys Lue Trp Asn Ile Arg Glu Ile Pro Ile Lue Phe Lue Arg His Thr Tyr Thr Asn Thr Asn Thr Asn Trp Ile Trp Lue Cys Gln Lue Arg Tyr Lue Gln Glu Gln Glu Ser Arg Ser Arg Pro Arg Ala Cys Ser Ser Ser Lys Ala * * Lue Trp Lys Lue Trp Arg Lue Trp * Lue Arg Lys Lue Arg Gln Lue Arg Glu Lue Pro Phe Arg Tyr Lue Phe Cys Tyr Lue Phe Cys His Pro Phe Lys Lue Lue Val Lys Lue Phe Ile Ser Tyr Thr Asn Thr Asn Ser Asn Arg Lue Arg Phe Ile Arg Lys Lue Arg Ser Ile Gln Glu Gln Glu Ser Thr Cys Ser Arg Ser Gln Pro Arg Arg Lue Trp Lys Lue Arg * Lue Arg * Lue Arg Gln Lue Arg Glu Lue His Pro Cys Lue His Lue Lue His Ile His Ile His Arg His Pro Asn Ser His Ser Lue Pro Asn Gly Tyr Thr Thr Thr Ala Pro Lys Tyr Tyr Asp Thr Thr Ala Arg Ser Asp Thr Phe Ser Lue Ala Met Thr Ile Asp Ser Lue Lue Tyr Thr His Ser Thr Met Asn Arg Lue Tyr Met Ile * Ser Met Lue Thr Ala Ser His * Lue Glu Asn Gly Lys \n",
      "\n",
      "L L S I L Q S V R G S Q H * S L Y H I L K A I H R L R V C I Q  T V N S H R  A E R I A S R C S V V V L R S R S C V A V G  G V G V G V T V D V D V E E V E A G V  F S  L P  S P  S P  F P  S S G L A S G A R A F L F L Y A P  L A Y E P  P V G V G V G V A D E E L D E E L E G V A E E V A E E V A E G  F S  L P  F P  S P  S P  F P  S S R F A T G A G S G A A S L T F L F L  V P  L A  L P Y P V G V G V G V G V T E E E D G Y F S Y V P  F P  S P  S P  S P  S A G E A S A L A S G * G S A T G A A T A P T A A N A K S I I K S L E K S I L N V I C L   I P G I P D R N C Q V L \n",
      "Lue Lue Ser Ile Lue Gln Ser Val Arg Gly Ser Gln His * Ser Lue Tyr His Ile Lue Lys Ala Ile His Arg Lue Arg Val Cys Ile Gln Thr Val Asn Ser His Arg Ala Glu Arg Ile Ala Ser Arg Cys Ser Val Val Val Lue Arg Ser Arg Ser Cys Val Ala Val Gly Gly Val Gly Val Gly Val Thr Val Asp Val Asp Val Glu Glu Val Glu Ala Gly Val Phe Ser Lue Pro Ser Pro Ser Pro Phe Pro Ser Ser Gly Lue Ala Ser Gly Ala Arg Ala Phe Lue Phe Lue Tyr Ala Pro Lue Ala Tyr Glu Pro Pro Val Gly Val Gly Val Gly Val Ala Asp Glu Glu Lue Asp Glu Glu Lue Glu Gly Val Ala Glu Glu Val Ala Glu Glu Val Ala Glu Gly Phe Ser Lue Pro Phe Pro Ser Pro Ser Pro Phe Pro Ser Ser Arg Phe Ala Thr Gly Ala Gly Ser Gly Ala Ala Ser Lue Thr Phe Lue Phe Lue Val Pro Lue Ala Lue Pro Tyr Pro Val Gly Val Gly Val Gly Val Gly Val Thr Glu Glu Glu Asp Gly Tyr Phe Ser Tyr Val Pro Phe Pro Ser Pro Ser Pro Ser Pro Ser Ala Gly Glu Ala Ser Ala Lue Ala Ser Gly * Gly Ser Ala Thr Gly Ala Ala Thr Ala Pro Thr Ala Ala Asn Ala Lys Ser Ile Ile Lys Ser Lue Glu Lys Ser Ile Lue Asn Val Ile Cys Lue Ile Pro Gly Ile Pro Asp Arg Asn Cys Gln Val Lue \n",
      "\n",
      "F Y P F S N Q C E A V N I D H Y I I Y  R R F I V  E C V Y N K L S I V I A K L N V S L R A V V S  Y L G A V V V  P L G R E W E L G * R W M W M W R R W R Q G C S S R S C R N H R S H R S F H S L L G W L L E H V L S C S C M L R S L R M N R N R L E L V L V  L M K S L T R S L K G W Q K R  Q K R  R K G S S R S C R S F R S H H S L H S F H N H H A L L L E Q A L G R L L  L S C S C R Y R S W H S Y H I Q L V L V L V  V * R R K R M G I S R M F H S F R N R H S H R S R H S P L E K H Q L W L L V E A L Q Q V Q R Q R Q R R R M P K A   S L L R K A Y * M  F V C S R S R E F R T E T V K S  \n",
      "Phe Tyr Pro Phe Ser Asn Gln Cys Glu Ala Val Asn Ile Asp His Tyr Ile Ile Tyr Arg Arg Phe Ile Val Glu Cys Val Tyr Asn Lys Lue Ser Ile Val Ile Ala Lys Lue Asn Val Ser Lue Arg Ala Val Val Ser Tyr Lue Gly Ala Val Val Val Pro Lue Gly Arg Glu Trp Glu Lue Gly * Arg Trp Met Trp Met Trp Arg Arg Trp Arg Gln Gly Cys Ser Ser Arg Ser Cys Arg Asn His Arg Ser His Arg Ser Phe His Ser Lue Lue Gly Trp Lue Lue Glu His Val Lue Ser Cys Ser Cys Met Lue Arg Ser Lue Arg Met Asn Arg Asn Arg Lue Glu Lue Val Lue Val Lue Met Lys Ser Lue Thr Arg Ser Lue Lys Gly Trp Gln Lys Arg Gln Lys Arg Arg Lys Gly Ser Ser Arg Ser Cys Arg Ser Phe Arg Ser His His Ser Lue His Ser Phe His Asn His His Ala Lue Lue Lue Glu Gln Ala Lue Gly Arg Lue Lue Lue Ser Cys Ser Cys Arg Tyr Arg Ser Trp His Ser Tyr His Ile Gln Lue Val Lue Val Lue Val Val * Arg Arg Lys Arg Met Gly Ile Ser Arg Met Phe His Ser Phe Arg Asn Arg His Ser His Arg Ser Arg His Ser Pro Lue Glu Lys His Gln Lue Trp Lue Lue Val Glu Ala Lue Gln Gln Val Gln Arg Gln Arg Gln Arg Arg Arg Met Pro Lys Ala Ser Lue Lue Arg Lys Ala Tyr * Met Phe Val Cys Ser Arg Ser Arg Glu Phe Arg Thr Glu Thr Val Lys Ser \n",
      "\n",
      "F I H S P I S A R Q S T L I I I S Y I K G D S S S K S V Y T I N C Q  S S L S * T Y R F A L  C R S T  E P  L C S R W V G S G S W G D G G C G C G G G G G R G V V L V V A V I T V V T V V S I V F W V G F W S T C F L V L V C S V A C V * T V T G W S W C W C S * * R A * R G A * R G G R R G S R R G S G R V V L V V A V V S V V T I V S I V S I I I T L C Y W S R L W G G F S N F L V L V G T V A G I A T I S S W C W C W C R C D G G R G W V F L V C S I V S V I A I V T V V A I V R W R S I S F G F W L R L C N R C S D S A N G G E C Q K H N K V S * E K H I E C N L F V V D P G N S G P K L S S P K \n",
      "Phe Ile His Ser Pro Ile Ser Ala Arg Gln Ser Thr Lue Ile Ile Ile Ser Tyr Ile Lys Gly Asp Ser Ser Ser Lys Ser Val Tyr Thr Ile Asn Cys Gln Ser Ser Lue Ser * Thr Tyr Arg Phe Ala Lue Cys Arg Ser Thr Glu Pro Lue Cys Ser Arg Trp Val Gly Ser Gly Ser Trp Gly Asp Gly Gly Cys Gly Cys Gly Gly Gly Gly Gly Arg Gly Val Val Lue Val Val Ala Val Ile Thr Val Val Thr Val Val Ser Ile Val Phe Trp Val Gly Phe Trp Ser Thr Cys Phe Lue Val Lue Val Cys Ser Val Ala Cys Val * Thr Val Thr Gly Trp Ser Trp Cys Trp Cys Ser * * Arg Ala * Arg Gly Ala * Arg Gly Gly Arg Arg Gly Ser Arg Arg Gly Ser Gly Arg Val Val Lue Val Val Ala Val Val Ser Val Val Thr Ile Val Ser Ile Val Ser Ile Ile Ile Thr Lue Cys Tyr Trp Ser Arg Lue Trp Gly Gly Phe Ser Asn Phe Lue Val Lue Val Gly Thr Val Ala Gly Ile Ala Thr Ile Ser Ser Trp Cys Trp Cys Trp Cys Arg Cys Asp Gly Gly Arg Gly Trp Val Phe Lue Val Cys Ser Ile Val Ser Val Ile Ala Ile Val Thr Val Val Ala Ile Val Arg Trp Arg Ser Ile Ser Phe Gly Phe Trp Lue Arg Lue Cys Asn Arg Cys Ser Asp Ser Ala Asn Gly Gly Glu Cys Gln Lys His Asn Lys Val Ser * Glu Lys His Ile Glu Cys Asn Lue Phe Val Val Asp Pro Gly Asn Ser Gly Pro Lys Lue Ser Ser Pro Lys \n",
      "\n",
      "Frame 1\n",
      "['ATGCTTTTCTCAAGAGACTTTATTATGCTTTTGGCATTCGCCGCCGTTGGCGCTGTCGCTGCACCTGTTGCAGAGCCTCAACCAGAAGCCAAAGCTGATGCTTCTCCAGCGGACTATGGCGACTACGGTGACTATGGCGATTACGGAAACTATGGAACATACGAGAAATACCCATCCTCTTCCTCCGTCACACCTACACCAACACCAACACCAACTGGATATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAGCAAAGCGTGATGATTATGGAAACTATGGAGACTATGGTGACTACGGAAACTACGGCAACTACGAGAACTACCCTTCCGCTACCTCTTCTGCTACCTCTTCTGCCACCCCTTCAAGCTCCTCGTCAAGCTCTTCATCAGCTACACCAACACCAACTCCAACCGGTTACGGTTCATACGCAAGCTACGGAGCATACAAGAACAAGAAAGCACGTGCTCCAGAAGCCAACCCAGAAGACTATGGAAACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTGCCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTACCCAACGGCTACACAACTACGGCTCCTAAGTACTACGACACTACAGCGCGAAGCGATACGTTCAGCTTAG', 'ATGCTTTTGGCATTCGCCGCCGTTGGCGCTGTCGCTGCACCTGTTGCAGAGCCTCAACCAGAAGCCAAAGCTGATGCTTCTCCAGCGGACTATGGCGACTACGGTGACTATGGCGATTACGGAAACTATGGAACATACGAGAAATACCCATCCTCTTCCTCCGTCACACCTACACCAACACCAACACCAACTGGATATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAGCAAAGCGTGATGATTATGGAAACTATGGAGACTATGGTGACTACGGAAACTACGGCAACTACGAGAACTACCCTTCCGCTACCTCTTCTGCTACCTCTTCTGCCACCCCTTCAAGCTCCTCGTCAAGCTCTTCATCAGCTACACCAACACCAACTCCAACCGGTTACGGTTCATACGCAAGCTACGGAGCATACAAGAACAAGAAAGCACGTGCTCCAGAAGCCAACCCAGAAGACTATGGAAACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTGCCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTACCCAACGGCTACACAACTACGGCTCCTAAGTACTACGACACTACAGCGCGAAGCGATACGTTCAGCTTAG', 'ATGGATAAA']\n",
      "Frame 2\n",
      "['ATGCTTCTCCAGCGGACTATGGCGACTACGGTGACTATGGCGATTACGGAAACTATGGAACATACGAGAAATACCCATCCTCTTCCTCCGTCACACCTACACCAACACCAACACCAACTGGATATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAG', 'ATGGCGACTACGGTGACTATGGCGATTACGGAAACTATGGAACATACGAGAAATACCCATCCTCTTCCTCCGTCACACCTACACCAACACCAACACCAACTGGATATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAG', 'ATGGCGATTACGGAAACTATGGAACATACGAGAAATACCCATCCTCTTCCTCCGTCACACCTACACCAACACCAACACCAACTGGATATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAG', 'ATGGAACATACGAGAAATACCCATCCTCTTCCTCCGTCACACCTACACCAACACCAACACCAACTGGATATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAG', 'ATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAG', 'ATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAG', 'ATGATTATGGAAACTATGGAGACTATGGTGACTACGGAAACTACGGCAACTACGAGAACTACCCTTCCGCTACCTCTTCTGCTACCTCTTCTGCCACCCCTTCAAGCTCCTCGTCAAGCTCTTCATCAGCTACACCAACACCAACTCCAACCGGTTACGGTTCATACGCAAGCTACGGAGCATACAAGAACAAGAAAGCACGTGCTCCAGAAGCCAACCCAGAAGACTATGGAAACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTGCCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTACCCAACGGCTACACAACTACGGCTCCTAA', 'ATGGAAACTATGGAGACTATGGTGACTACGGAAACTACGGCAACTACGAGAACTACCCTTCCGCTACCTCTTCTGCTACCTCTTCTGCCACCCCTTCAAGCTCCTCGTCAAGCTCTTCATCAGCTACACCAACACCAACTCCAACCGGTTACGGTTCATACGCAAGCTACGGAGCATACAAGAACAAGAAAGCACGTGCTCCAGAAGCCAACCCAGAAGACTATGGAAACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTGCCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTACCCAACGGCTACACAACTACGGCTCCTAA', 'ATGGAGACTATGGTGACTACGGAAACTACGGCAACTACGAGAACTACCCTTCCGCTACCTCTTCTGCTACCTCTTCTGCCACCCCTTCAAGCTCCTCGTCAAGCTCTTCATCAGCTACACCAACACCAACTCCAACCGGTTACGGTTCATACGCAAGCTACGGAGCATACAAGAACAAGAAAGCACGTGCTCCAGAAGCCAACCCAGAAGACTATGGAAACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTGCCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTACCCAACGGCTACACAACTACGGCTCCTAA', 'ATGGTGACTACGGAAACTACGGCAACTACGAGAACTACCCTTCCGCTACCTCTTCTGCTACCTCTTCTGCCACCCCTTCAAGCTCCTCGTCAAGCTCTTCATCAGCTACACCAACACCAACTCCAACCGGTTACGGTTCATACGCAAGCTACGGAGCATACAAGAACAAGAAAGCACGTGCTCCAGAAGCCAACCCAGAAGACTATGGAAACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTGCCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTACCCAACGGCTACACAACTACGGCTCCTAA', 'ATGGAAACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTGCCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTACCCAACGGCTACACAACTACGGCTCCTAA', 'ATGATCAATGTTGACTGCCTCGCACTGATTGGAGAATGGATAAAA']\n",
      "Frame 3\n",
      "['ATGACTATTGACAGTTTATTGTATACACACTCTTAG', 'ATGAATCGCCTTTAA', 'ATGATATAA', 'ATGTTGACTGCCTCGCACTGA', '']\n",
      "Frame 4\n",
      "['']\n",
      "Frame 5\n",
      "['ATGTGGATGTGGAGGAGGTGGAGGCAGGGGTGTAGTTCTCGTAGTTGCCGTAATCACCGTAGTCACCGTAGTTTCCATAGTCTTCTGGGTTGGCTTCTGGAGCACGTGCTTTCTTGTTCTTGTATGCTCCGTAGCTTGCGTATGAACCGTAACCGGTTGGAGTTGGTGTTGGTGTAG', 'ATGTGGAGGAGGTGGAGGCAGGGGTGTAGTTCTCGTAGTTGCCGTAATCACCGTAGTCACCGTAGTTTCCATAGTCTTCTGGGTTGGCTTCTGGAGCACGTGCTTTCTTGTTCTTGTATGCTCCGTAGCTTGCGTATGAACCGTAACCGGTTGGAGTTGGTGTTGGTGTAG', 'ATGCTCCGTAGCTTGCGTATGAACCGTAACCGGTTGGAGTTGGTGTTGGTGTAG', 'ATGAACCGTAACCGGTTGGAGTTGGTGTTGGTGTAG', 'ATGAAGAGCTTGACGAGGAGCTTGAAGGGGTGGCAGAAGAGGTAG', 'ATGGGTATTTCTCGTATGTTCCATAGTTTCCGTAATCGCCATAGTCACCGTAGTCGCCATAGTCCGCTGGAGAAGCATCAGCTTTGGCTTCTGGTTGAGGCTCTGCAACAGGTGCAGCGACAGCGCCAACGGCGGCGAATGCCAAAAGCATAA', 'ATGTTCCATAGTTTCCGTAATCGCCATAGTCACCGTAGTCGCCATAGTCCGCTGGAGAAGCATCAGCTTTGGCTTCTGGTTGAGGCTCTGCAACAGGTGCAGCGACAGCGCCAACGGCGGCGAATGCCAAAAGCATAA', 'ATGCCAAAAGCATAA', 'ATGTAA', '']\n",
      "Frame 6\n",
      "['']\n",
      "\n",
      "('Number 1 Translational ORF in Frame 1', 'ATGCTTTTCTCAAGAGACTTTATTATGCTTTTGGCATTCGCCGCCGTTGGCGCTGTCGCTGCACCTGTTGCAGAGCCTCAACCAGAAGCCAAAGCTGATGCTTCTCCAGCGGACTATGGCGACTACGGTGACTATGGCGATTACGGAAACTATGGAACATACGAGAAATACCCATCCTCTTCCTCCGTCACACCTACACCAACACCAACACCAACTGGATATGGTAGCTATGCCAGCTACGGTACCTACAAGAACAAGAAAGTTAGAGAAGCCGCCCCAGAGCCTGCTCCAGTAGCAAAGCGTGATGATTATGGAAACTATGGAGACTATGGTGACTACGGAAACTACGGCAACTACGAGAACTACCCTTCCGCTACCTCTTCTGCTACCTCTTCTGCCACCCCTTCAAGCTCCTCGTCAAGCTCTTCATCAGCTACACCAACACCAACTCCAACCGGTTACGGTTCATACGCAAGCTACGGAGCATACAAGAACAAGAAAGCACGTGCTCCAGAAGCCAACCCAGAAGACTATGGAAACTACGGTGACTACGGTGATTACGGCAACTACGAGAACTACACCCCTGCCTCCACCTCCTCCACATCCACATCCACCGTCACCCCAACTCCCACTCCCTACCCAACGGCTACACAACTACGGCTCCTAAGTACTACGACACTACAGCGCGAAGCGATACGTTCAGCTTAG')\n",
      "910 : 858\n"
     ]
    }
   ],
   "metadata": {}
  }
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