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Can xiSearch identify a mixture of 14N and 15N labelled xlinked peptides #94

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BioBern opened this issue Nov 27, 2023 · 0 comments
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@BioBern
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BioBern commented Nov 27, 2023

Can xiSearch handle the analysis of isotope labelled proteins? We mix normal protein with the 15N isotopic labelled version of the same protein. We get a mixture of light 14N peptides crosslinked with heavy 15N labelled peptides. I have both heavy 15N and light 14N peptides at the same time and L-L, L-H and H-H crosslinked peptides are present in the sample. Can I use xiSearch to detect these heavy and light xlinked peptides?

@Rappsilber-Laboratory Rappsilber-Laboratory locked and limited conversation to collaborators Nov 27, 2023
@lutzfischer lutzfischer converted this issue into discussion #95 Nov 27, 2023

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