Hello,
Thanks for coming up with such an easy-to-use handy tool. I have used polyester to generate reads by feeding it with fold change information. While most of the simulated data is perfect, I have a few comments on their format.
- No options for fastq. I have a software pipeline which assumes input in fastq format. It would be really great to have an option to output files in fastq format. Some fake quality values are also fine.
- The headers of the read contain a '/' character. This creates a problem for downstream applications since STAR eliminates all characters after '/'
- For single ended reads I found that the name of the reads contained the character 'mate'. i am not sure if that was due to a glitch in the code.
Hello,
Thanks for coming up with such an easy-to-use handy tool. I have used polyester to generate reads by feeding it with fold change information. While most of the simulated data is perfect, I have a few comments on their format.