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Methylcaller alternatives to recommended MethylDackel #67

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Blosberg opened this issue Sep 22, 2020 · 1 comment
Open

Methylcaller alternatives to recommended MethylDackel #67

Blosberg opened this issue Sep 22, 2020 · 1 comment

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@Blosberg
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Blosberg commented Sep 22, 2020

Thanks very much for providing bwa-meth to the community.

The README recommends using MethylDackel for methylation calling/tabulation, etc., however MethylDackel has had a critical error leading to a seg-fault/core-dump which, apparently, has been unresolved for a few months now. In looking for alternatives, I tried using mcall, but mcall log shows the following error:

[..]
XR:Z or ZR:Z:, or ZS:Z: field not found
[..] 

I know this isn't really your responsibility, but can you recommend an alternative methyl-caller package to use downstream of bwameth until the issue with MethylDackel is resolved? thanks for any advice you can offer.

@brentp
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brentp commented Sep 22, 2020

It looks like a solution there is to build with htslib 1.9 instead of relying on the conda build. (You could also try htslib 1.11 which is released today).
I don't know of other tools beyond MethylDackel.

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