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### Introduction
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- RNAseq is a bioinformatics analysis pipeline used for RNA sequencing data at the
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+ cellgeni/rnaseq is a bioinformatics analysis pipeline used for RNA sequencing data at the
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[ Cellular Genetics program] ( http://www.sanger.ac.uk/science/programmes/cellular-genetics )
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at [ the Wellcome Sanger Institute] ( http://www.sanger.ac.uk/ ) .
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@@ -59,7 +59,7 @@ orchestration.
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- We use the sample ID as the primary process ID and tag everywhere.
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- The building of indexes was split off into a separate file ` buildindex.nf ` . This file
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- still needs a lot of work.
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+ still needs a lot of work to recover from the splitting step .
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- All code was pulled out of conditional statements, enabling us to run different
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modes at the same time. This is helpful for testing, reduces overhead, and reduces
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### Diagram
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This is the flow chart of the pipeline.
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+ ![ cellgeni/rnaseq flowchart] ( flowchart.png )
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how did that work again?
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memory { reads.size() < 70.KB ? 1.GB : 5.GB }
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+ - dump everythign in https://www.nextflow.io/docs/latest/metadata.html
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- -m hisat2 for multiQC?
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- samtools collate has -f option for fast. Can we use this?
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