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I used star for alignment, and the fraction of mapped reads was 82.18%. But in RSEM expression files (genes.results and isoforms.results), the values of "expected_count", "TPM", "FPKM" and "IsoPct" columns are all zero.
Totally, I have four batches of RNA-seq data, only one batch of data encountered this error. And I used the same scripts for these four batches.
rsem-calculate-expression -p 12 --star --no-bam-output --paired-end --star-gzipped-read-file ~/trimmed.R1.fastq.gz ~/trimmed.R2.fastq.gz ~/rsem/star_index/star_index ~/RSEM/star_rsem/
The only difference I can think of between this batch and other batches is that it's data size is very large.
Any suggestions?
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