diff --git a/CHANGES.md b/CHANGES.md
index edb1efe0..5bd821f0 100644
--- a/CHANGES.md
+++ b/CHANGES.md
@@ -24,6 +24,7 @@
 -   The GDAL library included in the wheels is upgraded from 3.10.3 to 3.11.4 (#578).
 -   Add libkml driver to the wheels for more recent Linux platforms supported
     by manylinux_2_28, MacOS, and Windows (#561).
+-   Add libspatialite to the wheels (#546).
 -   Minimum required Python version is now 3.10 (#557).
 -   Wheels are now available for Python 3.14 (#579).
 
diff --git a/ci/vcpkg-manylinux2014.json b/ci/vcpkg-manylinux2014.json
index c8b96c2e..a19b1381 100644
--- a/ci/vcpkg-manylinux2014.json
+++ b/ci/vcpkg-manylinux2014.json
@@ -2,10 +2,17 @@
     "name": "pyogrio",
     "version": "0.12.0",
     "dependencies": [
+        {
+            "name": "libspatialite",
+            "default-features": false,
+            "features": [
+                "rttopo"
+            ]
+        },
         {
             "name": "gdal",
             "default-features": false,
-            "features": ["recommended-features", "curl", "geos", "iconv", "openssl"]
+            "features": ["recommended-features", "curl", "geos", "iconv", "libspatialite", "openssl"]
         }
     ],
     "builtin-baseline": "da096fdc67db437bee863ae73c4c12e289f82789"
diff --git a/ci/vcpkg.json b/ci/vcpkg.json
index 41f5259d..7796dbb0 100644
--- a/ci/vcpkg.json
+++ b/ci/vcpkg.json
@@ -2,11 +2,18 @@
     "name": "pyogrio",
     "version": "0.12.0",
     "dependencies": [
+        {
+            "name": "libspatialite",
+            "default-features": false,
+            "features": [
+                "rttopo"
+            ]
+        },
         {
             "name": "gdal",
             "default-features": false,
             "features": [
-                "recommended-features", "curl", "geos", "iconv", "libkml", "openssl"
+                "recommended-features", "curl", "geos", "iconv", "libkml", "libspatialite", "openssl"
             ]
         }
     ],
diff --git a/pyogrio/tests/test_geopandas_io.py b/pyogrio/tests/test_geopandas_io.py
index e4cad146..d2d4f31d 100644
--- a/pyogrio/tests/test_geopandas_io.py
+++ b/pyogrio/tests/test_geopandas_io.py
@@ -104,14 +104,11 @@ def use_arrow_context():
         del os.environ["PYOGRIO_USE_ARROW"]
 
 
-def spatialite_available(path):
-    try:
-        _ = read_dataframe(
-            path, sql="select spatialite_version();", sql_dialect="SQLITE"
-        )
-        return True
-    except Exception:
-        return False
+def test_spatialite_available(test_gpkg_nulls):
+    """Check if SpatiaLite is available by running a simple SQL query."""
+    _ = read_dataframe(
+        test_gpkg_nulls, sql="select spatialite_version();", sql_dialect="SQLITE"
+    )
 
 
 @pytest.mark.parametrize(