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Plots not displaying Abundance % #195

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kiranpatil222 opened this issue Mar 19, 2025 · 0 comments
Open

Plots not displaying Abundance % #195

kiranpatil222 opened this issue Mar 19, 2025 · 0 comments

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@kiranpatil222
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kiranpatil222 commented Mar 19, 2025

Hi have installed and build Db from the below link tutorial.

https://www.youtube.com/watch?v=In56LDyNbpI
https://github.com/vappiah/bioinformatics-tutorials/tree/main/software-installation/krona-tools

and i ran below commands

kraken2 --db Kraken2_Db --threads 6 --use-names --report kraken2_report.txt --output kraken2_output.txt 23.fastq
bracken -d Kraken2_Db -i kraken2_report.txt -o bracken_output.txt -r 300 -l S
ktImportTaxonomy bracken_output.txt -o B07_Krona.html

its generating HTML report, but my sample has 70% EBOLA species as per krona and braken , but in HTML report its not reflecting EBOLA virus as majority instead its show root and bacterial abundances like 0-1-3% of different species though my braken kraken has 3 different roots , bacteria, cellular and Viral, Eukaryotes.

Is there anything i am doing wrong or if anything or build database wrong,, Please, I am new to Krona Plots.

Thanks

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