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Using meryl to prepare for Merqury #24

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aureliendejode opened this issue Jul 15, 2021 · 2 comments
Open

Using meryl to prepare for Merqury #24

aureliendejode opened this issue Jul 15, 2021 · 2 comments

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@aureliendejode
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Hi,

I'd like to evaluate my assembly. I have several Illumina fastq files. For each library I have one fastq file for Forward and one for Reverse reads.
What is the best way to use Meryl in the goal of using that output for Merqury:
Is something like this okay:
meryl count k=21 lib1_1.fastq lib1_2.fastq lib2_1.fastq lib2_2.fastq ... output Illumina.meryl

Thanks

@brianwalenz
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That will work.

You might want to limit memory and thread usage with memory=<limit in GB> and threads=<number of CPUs to use>. By default, it will use as much memory as it wants and all the CPUs.

@aureliendejode
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Thanks, I used those 2 options and it worked.

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