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Design DNA Metabarcode to Analyze Wolf Diet

Learning goals
  • Set up computer environment with bash
  • Install OBITools, ecoPCR and ecoPrimers with bash
  • Install ROBITools and related packages in R
  • Download complete mitochondrial genomes from NCBI ftp files
  • Streamline the computation process
  • DNA metabarcode design strategy
  • DNA metabarcode selection criteria
  • Blocking primer design