how to do Step4, when i only have multiple independent mrc files?

[yahulai2014]

To convert multiple independent MRC files into MRC stack files??? and how to do it/? '--data_path T00_HA_130K-Equalized-Particle-Stack**.mrcs** '

[zhanghui186]

To create a particle stack, you can use the relion_stack_create command. Here are the specific instructions:
“relion_stack_create --i particles.star --o filename”
In this command, particles.star is the STAR file that contains all the particles. If you have performed preprocessing in cryoSPARC, you can use the "pyem" tool to convert the CS file to a STAR file before running the command.