diff --git a/scripts/create_test_resources/2023_10x_mouse_brain_xenium_rep1.sh b/scripts/create_test_resources/2023_10x_mouse_brain_xenium_rep1.sh index b48d1b01d..519e19b1a 100755 --- a/scripts/create_test_resources/2023_10x_mouse_brain_xenium_rep1.sh +++ b/scripts/create_test_resources/2023_10x_mouse_brain_xenium_rep1.sh @@ -8,8 +8,8 @@ cd "$REPO_ROOT" set -e -if [ ! -d temp/datasets/10x_xenium/2023_10x_mouse_brain_xenium ]; then - mkdir -p temp/datasets/10x_xenium/2023_10x_mouse_brain_xenium +if [ ! -d temp/datasets/10x_xenium/2023_10x_mouse_brain_xenium_rep1 ]; then + mkdir -p temp/datasets/10x_xenium/2023_10x_mouse_brain_xenium_rep1 fi if [ ! -f temp/datasets/10x_xenium/2023_10x_mouse_brain_xenium_rep1/Xenium_V1_FF_Mouse_Brain_MultiSection_1_outs.zip ]; then wget -O temp/datasets/10x_xenium/2023_10x_mouse_brain_xenium_rep1/Xenium_V1_FF_Mouse_Brain_MultiSection_1_outs.zip \ @@ -29,9 +29,9 @@ param_list: dataset_description: Demonstration of gene expression profiling for fresh frozen mouse brain on the Xenium platform using the pre-designed Mouse Brain Gene Expression Panel (v1). dataset_organism: mus_musculus crop_region_min_x: 10000 - crop_region_max_x: 12000 + crop_region_max_x: 11000 crop_region_min_y: 10000 - crop_region_max_y: 12000 + crop_region_max_y: 11000 publish_dir: resources_test/common output_dataset: '\$id/dataset.zarr' diff --git a/scripts/create_test_resources/test_pipeline.sh b/scripts/create_test_resources/test_pipeline.sh index 538cdc467..6ffba9110 100755 --- a/scripts/create_test_resources/test_pipeline.sh +++ b/scripts/create_test_resources/test_pipeline.sh @@ -12,6 +12,13 @@ mkdir -p $OUT_DIR # run dataset preprocessor viash run src/data_processors/process_dataset/config.vsh.yaml -- \ + --dataset_id mouse_brain_combined \ + --dataset_name "Test data mouse brain combined 2023 tenx Xenium replicate 1 2023 Yao scRNAseq" \ + --dataset_url "https://www.10xgenomics.com/datasets/fresh-frozen-mouse-brain-replicates-1-standard;https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE246717" \ + --dataset_reference "https://www.10xgenomics.com/datasets/fresh-frozen-mouse-brain-replicates-1-standard;10.1038/s41586-023-06812-z" \ + --dataset_summary "Demonstration of gene expression profiling for fresh frozen mouse brain on the Xenium platform using the pre-designed Mouse Brain Gene Expression Panel (v1);A high-resolution scRNAseq atlas of cell types in the whole mouse brain" \ + --dataset_description "Demonstration of gene expression profiling for fresh frozen mouse brain on the Xenium platform using the pre-designed Mouse Brain Gene Expression Panel (v1). Replicate results demonstrate the high reproducibility of data generated by the platform. 10x Genomics obtained tissue from a C57BL/6 mouse from Charles River Laboratories. Three adjacent 10µm sections were placed on the same slide. Tissues were prepared following the demonstrated protocols Xenium In Situ for Fresh Frozen Tissues - Tissue Preparation Guide (CG000579) and Xenium In Situ for Fresh Frozen Tissues - Fixation & Permeabilization (CG000581).;See dataset_reference for more information. Note that we only took the 10xv2 data from the dataset." \ + --dataset_organism "mus_musculus" \ --input_sc $SC_DIR/dataset.h5ad \ --input_sp $SP_DIR/dataset.zarr \ --output_sc $OUT_DIR/scrnaseq_reference.h5ad \ diff --git a/src/datasets/loaders/tenx_xenium/config.vsh.yaml b/src/datasets/loaders/tenx_xenium/config.vsh.yaml index f16032a9b..d8a1dd367 100644 --- a/src/datasets/loaders/tenx_xenium/config.vsh.yaml +++ b/src/datasets/loaders/tenx_xenium/config.vsh.yaml @@ -67,4 +67,4 @@ runners: - type: executable - type: nextflow directives: - label: [highmem, midcpu, midtime] + label: [midmem, midcpu, midtime] diff --git a/src/datasets/workflows/process_tenx_xenium/config.vsh.yaml b/src/datasets/workflows/process_tenx_xenium/config.vsh.yaml index e9507cb65..c4fec772b 100644 --- a/src/datasets/workflows/process_tenx_xenium/config.vsh.yaml +++ b/src/datasets/workflows/process_tenx_xenium/config.vsh.yaml @@ -82,4 +82,4 @@ dependencies: runners: - type: nextflow directives: - label: [highcpu, highmem, hightime] + label: [highcpu, midmem, hightime] diff --git a/src/datasets/workflows/process_wu_human_breast_cancer_sc/config.vsh.yaml b/src/datasets/workflows/process_wu_human_breast_cancer_sc/config.vsh.yaml index 93c33e23b..c0fdf6f52 100644 --- a/src/datasets/workflows/process_wu_human_breast_cancer_sc/config.vsh.yaml +++ b/src/datasets/workflows/process_wu_human_breast_cancer_sc/config.vsh.yaml @@ -88,8 +88,10 @@ dependencies: repository: openproblems - name: datasets/processors/knn repository: openproblems - - name: h5ad/extract_uns_metadata - repository: core + - name: utils/extract_uns_metadata + repository: openproblems +# - name: h5ad/extract_uns_metadata +# repository: core runners: - type: nextflow