diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index a4489e609..9f4646e74 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -155,7 +155,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python: ['3.9', '3.10', '3.11', '3.12', '3.13']
+        python: ['3.9', '3.10', '3.11', '3.12', '3.13', '3.14']
         arch: ['x86', 'x64']
         lsp: ['']
         lsp_extract_file: ['']
@@ -237,7 +237,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python: ['pypy-3.10', '3.9', '3.10', '3.11', '3.12', '3.13']
+        python: ['pypy-3.10', '3.9', '3.10', '3.11', '3.12', '3.13', '3.14']
         check_formatting: ['0']
         no_test_requirements: ['0']
         extra_name: ['']
@@ -301,7 +301,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python: ['pypy-3.10', '3.9', '3.10', '3.11', '3.12', '3.13']
+        python: ['pypy-3.10', '3.9', '3.10', '3.11', '3.12', '3.13', '3.14']
     continue-on-error: >-
       ${{
         (
diff --git a/src/trio/_core/_tests/test_run.py b/src/trio/_core/_tests/test_run.py
index 289a35667..7728a6f3d 100644
--- a/src/trio/_core/_tests/test_run.py
+++ b/src/trio/_core/_tests/test_run.py
@@ -2817,6 +2817,10 @@ def no_other_refs() -> list[object]:
     sys.implementation.name != "cpython",
     reason="Only makes sense with refcounting GC",
 )
+@pytest.mark.xfail(
+    sys.version_info >= (3, 14),
+    reason="https://github.com/python/cpython/issues/125603",
+)
 async def test_ki_protection_doesnt_leave_cyclic_garbage() -> None:
     class MyException(Exception):
         pass
diff --git a/test-requirements.in b/test-requirements.in
index 2e29cda42..bf64c568d 100644
--- a/test-requirements.in
+++ b/test-requirements.in
@@ -12,7 +12,7 @@ cryptography>=41.0.0  # cryptography<41 segfaults on pypy3.10
 # Tools
 black; implementation_name == "cpython"
 mypy  # Would use mypy[faster-cache], but orjson has build issues on pypy
-orjson; implementation_name == "cpython"
+orjson; implementation_name == "cpython" and python_version < "3.14"    # orjson does not yet install on 3.14
 ruff >= 0.8.0
 astor          # code generation
 uv >= 0.2.24
diff --git a/test-requirements.txt b/test-requirements.txt
index 2865e4c4a..8a53682a6 100644
--- a/test-requirements.txt
+++ b/test-requirements.txt
@@ -92,7 +92,7 @@ nodeenv==1.9.1
     # via
     #   pre-commit
     #   pyright
-orjson==3.10.15 ; implementation_name == 'cpython'
+orjson==3.10.15 ; python_full_version < '3.14' and implementation_name == 'cpython'
     # via -r test-requirements.in
 outcome==1.3.0.post0
     # via -r test-requirements.in