Y26-104 - Bed Verification for SPRI on Hamilton "Porkins" and "Hamlet" Post Amplification AA209

[Stan1520]

Context
The current SPRI protocol in Post Amplification AA209 does not include bed verification in LIMBER. This removes an important quality control checkpoint between SPRI and PCR steps, increasing the risk of historical quality issues reoccurring when the process is brought back online. Bed verification is required between defined protocol steps to safeguard the process.

User story
As a laboratory operator, I want bed verification checkpoints added to the SPRI workflow in LIMBER for Hamilton "Porkins" and "Hamlet" in Post Amplification AA209, So that plate positioning is verified between SPRI and PCR steps to prevent process errors and protect sample quality.

Who are the primary contacts for this story
Joanna C

Who is the nominated tester for UAT
Joanna C

Acceptance criteria
To be considered successful the solution must allow:

• Add new bed verification LRC GEM-X 5p GE LigXP --> LRC GEM-X 5p GE PCR 2XP
• bed 7: LRC GEM-X 5p GE LigXP
• bed 10: LRC GEM-X 5p GE PCR 2XP

[KatyTaylor]

Which Limber pipeline are we talking about here? See https://limber.psd.sanger.ac.uk/pipelines?group=true for the whole list.

Can you describe the relevant transfer in terms of source and target plate purposes (also shown on the above diagram)?

What are the details of the bed verification i.e. which plate purposes should be on which beds?

[me12-WSI]

Hi Katy,

Pipelines:

• scRNA Core Library Prep
• scRNA Core BCR Library Prep
• scRNA Core TCR Library Prep

I've attached pictures of which steps these go between

[KatyTaylor]

@me12-WSI That's great thank you. Could you also please provide, for each of the 3 bed verifications:

• Source plate bed number(s)
• Destination plate bed number(s)

[Stan1520]

Refinement Notes: 31/03/2026
Meg to come back on the story with details of the bed verification i.e. which plate purposes should be on which beds?, Robot type,

Then Sized as a small

[KatyTaylor]

Update: checked the existing bed verifications in Limber config and found the following. So they seem to be present already. Are they not working?

hamilton LRC GEM-X 5p TCR Post Lig 1XSPRI => LRC GEM-X 5p TCR Post PCR

bed 7: LRC GEM-X 5p TCR Post Lig 1XSPRI
bed 10: LRC GEM-X 5p TCR Post PCR

hamilton LRC GEM-X 5p BCR Post Lig 1XSPRI => LRC GEM-X 5p BCR Post PCR

bed 7: LRC GEM-X 5p BCR Post Lig 1XSPRI
bed 10: LRC GEM-X 5p BCR Post PCR

However: there is no existing bed verification for the LRC GEM-X 5p GE LigXP --> LRC GEM-X 5p GE PCR 2XP step, so we can add one here.

[me12-WSI]

Hi,

Apologies for the slow reply! The beds are the same for GEX so:
bed 7: LRC GEM-X 5p GE LigXP
bed 10: LRC GEM-X 5p GE PCR 2XP

This line is not currently running so I can't check if the BCR/TCR verifications are working

[KatyTaylor]

Hi,

Apologies for the slow reply! The beds are the same for GEX so: bed 7: LRC GEM-X 5p GE LigXP bed 10: LRC GEM-X 5p GE PCR 2XP

This line is not currently running so I can't check if the BCR/TCR verifications are working

Thanks @me12-WSI
I have updated the acceptance criteria to add the bed verification step for the Gene Expression branch. Will you be able to test it for us (when ready) it if the line isn't running? I guess you wouldn't use real plates anyway, just click through in the UAT environment with fake plates.

[sambucaboywicked]

Hi,

Yeah I would push it through UAT with fake plates. Thanks!