@@ -11,15 +11,15 @@ The dataset for reproducing this tutorial can be obtained from
1111This tutorial utilizes 2 raw data files (* .raw) from https://repository.jpostdb.org/entry/JPST000200 .
1212The human_proteins_ref.fasta was obtained from https://www.uniprot.org/ .
1313
14- ### 1-1 Starting up your project
14+ ### 1-2 Starting up your project
1515
1616![ Figure 1: Starting up a project in MS-DIAL5. First, open the
1717MSDIAL.exe file in the downloaded folder (1). Click on “New
1818project” (2). You can name your project according to your preferences.
1919Browse to the location of your experimental files. Click next and then
2020continue to the second left side bar “Raw measurement files”. Here,
2121click browse to access your raw data files.]
22- (images/proteomics/Fig1%20Starting%20up%20a%20project.png){#fig-1}
22+ (images/proteomics/Fig1%20Starting%20up%20a%20project.png)
2323
2424![ Figure 2: Importing raw data files and setting up the measurement
2525parameters. Change the type of file according to your vendor format
@@ -31,8 +31,7 @@ batch, analytical order, dilution factor, or possibly exclude some
3131samples from further data processing (2, 3). This is because to be able
3232to use all MS-DIAL functions. After clicking next and selecting the
3333“Measurement parameters” in the left side tab, you can specify your
34- analytical setup according to this figure (4).]
35- (images/proteomics/Fig2%20Improting%20a%20raw%20data%20files%20and%20setting%20up%20parameters.png){#fig-2}
34+ analytical setup according to this figure (4).] ( images/proteomics/Fig2%20Improting%20a%20raw%20data%20files%20and%20setting%20up%20parameters.png )
3635
3736![ Figure 3: Setting other project parameters according to your thoughts.
3837Specifically, in the “Identification” section, FASTA files obtained from
@@ -41,8 +40,7 @@ Uniprot or other sources can be set as a database. Select the “+” next to
4140your files (Fasta) (2). In addition to this, if you browse the enzyme setting,
4241you can select the enzyme according to your experimental setup (3, 4). Also,
4342you can set modification settings in this entry. In the proteomics mode of
44- MS-DIAL, carbamidomethyl (CAM) of cysteine is considered in the default settings.]
45- (images/proteomics/Fig3%20Setting%20up%20analysis%20parameters.png){#fig-3}
43+ MS-DIAL, carbamidomethyl (CAM) of cysteine is considered in the default settings.] ( images/proteomics/Fig3%20Setting%20up%20analysis%20parameters.png )
4644
4745![ Figure 4: This is the main view of MS-DIAL. Double-clicking on a file name
4846in the file navigator will display the detected peak information in the center
@@ -57,8 +55,7 @@ tandem mass spectrum. Assuming peptide bond cleavage induced by Collision Induce
5755Dissociation (CID), lists of b-ions and y-ions derived from each peptide sequence
5856are generated and compared. In MS-DIAL, product ions with charges greater than one
5957are also considered. B-ions are displayed in red and y-ions in cyan, shown as paired
60- spectra below the measured spectrum (6).]
61- (images/proteomics/Fig4%20Project%20windows%20after%20analysis.png){#fig-4}
58+ spectra below the measured spectrum (6).] ( images/proteomics/Fig4%20Project%20windows%20after%20analysis.png )
6259
6360The video tutorial for the MS-DAIL5 operation
6461
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