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proteomics.qmd

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@@ -11,15 +11,15 @@ The dataset for reproducing this tutorial can be obtained from
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This tutorial utilizes 2 raw data files (*.raw) from https://repository.jpostdb.org/entry/JPST000200.
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The human_proteins_ref.fasta was obtained from https://www.uniprot.org/.
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### 1-1 Starting up your project
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### 1-2 Starting up your project
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![Figure 1: Starting up a project in MS-DIAL5. First, open the
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MSDIAL.exe file in the downloaded folder (1). Click on “New
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project” (2). You can name your project according to your preferences.
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Browse to the location of your experimental files. Click next and then
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continue to the second left side bar “Raw measurement files”. Here,
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click browse to access your raw data files.]
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(images/proteomics/Fig1%20Starting%20up%20a%20project.png){#fig-1}
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(images/proteomics/Fig1%20Starting%20up%20a%20project.png)
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![Figure 2: Importing raw data files and setting up the measurement
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parameters. Change the type of file according to your vendor format
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samples from further data processing (2, 3). This is because to be able
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to use all MS-DIAL functions. After clicking next and selecting the
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“Measurement parameters” in the left side tab, you can specify your
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analytical setup according to this figure (4).]
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(images/proteomics/Fig2%20Improting%20a%20raw%20data%20files%20and%20setting%20up%20parameters.png){#fig-2}
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analytical setup according to this figure (4).](images/proteomics/Fig2%20Improting%20a%20raw%20data%20files%20and%20setting%20up%20parameters.png)
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![Figure 3: Setting other project parameters according to your thoughts.
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Specifically, in the “Identification” section, FASTA files obtained from
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your files (Fasta) (2). In addition to this, if you browse the enzyme setting,
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you can select the enzyme according to your experimental setup (3, 4). Also,
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you can set modification settings in this entry. In the proteomics mode of
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MS-DIAL, carbamidomethyl (CAM) of cysteine is considered in the default settings.]
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(images/proteomics/Fig3%20Setting%20up%20analysis%20parameters.png){#fig-3}
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MS-DIAL, carbamidomethyl (CAM) of cysteine is considered in the default settings.](images/proteomics/Fig3%20Setting%20up%20analysis%20parameters.png)
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![Figure 4: This is the main view of MS-DIAL. Double-clicking on a file name
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in the file navigator will display the detected peak information in the center
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Dissociation (CID), lists of b-ions and y-ions derived from each peptide sequence
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are generated and compared. In MS-DIAL, product ions with charges greater than one
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are also considered. B-ions are displayed in red and y-ions in cyan, shown as paired
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spectra below the measured spectrum (6).]
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(images/proteomics/Fig4%20Project%20windows%20after%20analysis.png){#fig-4}
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spectra below the measured spectrum (6).](images/proteomics/Fig4%20Project%20windows%20after%20analysis.png)
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The video tutorial for the MS-DAIL5 operation
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