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25 | 25 |
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26 | 26 | \title[StainedGlass]{StainedGlass: Making colorful dot-plots of genomic sequence}
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27 | 27 | \author[Vollger \textit{et~al}.]{
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28 |
| - Mitchell R. Vollger\,$^{\text{\sfb 1,}*}$, |
| 28 | + Mitchell R. Vollger\,$^{\text{\sfb 1,}}$, |
29 | 29 | Peter Kerpedjiev\,$^{\text{\sfb 2}}$,
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30 |
| - Adam M. Phillippy\,$^{\text{\sfb 3,*}}$, |
| 30 | + Adam M. Phillippy\,$^{\text{\sfb 3,}}$, |
31 | 31 | and Evan E. Eichler\,$^{\text{\sfb 1,4,}*}$}
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32 | 32 | \address{
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33 | 33 | $^{\text{\sf 1}}$Genome Sciences,
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47 | 47 |
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48 | 48 | \abstract{
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49 | 49 | \textbf{Summary:}
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50 |
| -Visualization of genomic repeats is often accomplished through the use of |
51 |
| -dot plots; however, the emergence of telomere-to-telomere assemblies |
52 |
| -with multi-megabase repeats requires new visualization strategies. Here, |
53 |
| -we introduce StainedGlass which can generate publication quality figures |
54 |
| -that communicate the identity and orientation of |
55 |
| -multi-megabase repeats while scaling to entire genomes. \\ |
| 50 | +Visualization and analysis of genomic repeats is typically accomplished through the use of dot plots; however, the emergence of telomere-to-telomere assemblies with multi-megabase repeats requires new visualization strategies. Here, we introduce StainedGlass which can generate publication quality figures and interactive visualizations that depict the identity and orientation of multi-megabase repeat structures at a genome-wide scale. The tool can rapidly reveal higher-order structures and improve the inference of evolutionary history for some of the most complex regions of genomes. |
| 51 | +\\ |
56 | 52 | \textbf{Availability and implementation:}
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57 |
| -StainedGlass is implemented using \href{https://snakemake.github.io}{snakemake} |
58 |
| -and is available open source under the MIT license at |
59 |
| -\href{https://mrvollger.github.io/StainedGlass}{mrvollger.github.io/StainedGlass/}.\\ |
| 53 | +StainedGlass is implemented using \href{https://snakemake.github.io}{Snakemake} and is available open source under the MIT license at \href{https://mrvollger.github.io/StainedGlass}{mrvollger.github.io/StainedGlass/}.\\ |
60 | 54 |
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61 | 55 | }
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62 | 56 |
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@@ -93,7 +87,7 @@ \section{Methods}
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93 | 87 | %%--------------------------------------------------------------------------%%
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94 | 88 | \begin{figure*}[!tpb]%figure1
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95 | 89 | \centerline{
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96 |
| - \includegraphics[width=0.95\textwidth,keepaspectratio]{figure1.png} |
| 90 | + \includegraphics[width=0.95\textwidth,keepaspectratio,,natwidth=7200,natheight=4800]{figure1.png} |
97 | 91 | }
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98 | 92 | \caption{Output from StainedGlass showing the sequence identity within human
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99 | 93 | chromosome 8.}
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