eDNA-protocols

Protocols for sampling, processing, and analysis of eDNA collected on CalCOFI cruises as part of the West Coast OBON sampling effort. All protocols are formatted according to guidelines from Better Biomolecular Ocean Practices (BeBOP) using Minimum Information about an Omics Protocol (MIOP) and FAIR eDNA (FAIRe) terms.

Sample collection

RREAS sampling protocol from Niskin bottles

Sampling from ship's underway water system

Cleaning protocol for sampling materials

DNA Extraction

Extracting from membrane filters

Extracting from Sterivex

PCR (coming soon)