DNBelab_C_Series_HT_scRNA-analysis-software

An open source and flexible pipeline to analysis high-throughput DNBelab C Series single-cell RNA datasets

Introduction

• Propose
  • An open source and flexible pipeline to analyze DNBelab C Series^TM single-cell RNA datasets.
• Language
  • Python3(>=3.8.*),R scripts
• Hardware/Software requirements
  • x86-64 compatible processors.
  • require at least 50GB of RAM and 4 CPU.
  • centos 7.x 64-bit operating system (Linux kernel 3.10.0, compatible with higher software and hardware configuration).

Directory contents

• DNBC4tools config, software and script directories.
• doc Instruction for use.
• example Example of use the pipline.
• scripts Miscellaneous scripts.
• wdl WDL pipeline.

Installation

installation manual here

Database

Create database manual here

Start

Command line : Using the DNBC4tools

• DNBC4tools

Container

• Docker/Singularity

WDL : Customize the config file, use wdl to analysis

• WDL