MINPP1_reactionNetwork

this repository contains supporting information to the manuscript:
Nguyen Trung, M., Kieninger, S., ... B. G. Keller, Fiedler, D. (2022). "Stable isotopomers of myo-inositol to uncover the complex MINPP1-dependent inositol phosphate network", ACS Central Science, 8(12), 1683-1694.
https://doi.org/10.1021/acscentsci.2c01032

In our work, we numerically analyzed experimentally determined progress curves for the MINPP1 mediated dephosphorylation of InsP₅[2OH] and InsP₆. This repository contains the corresponding Python3 scripts that were used to extract the kinetic rates from the experimental data sets.

Overview

All analysis was performed with Python3. The files "fitted_exp_data.py" contain all fit functions that where used to fit the scaled experimental data. The files "minimization_routine.py"contain the code for the entire minimization routine including initial guess and reconstruction of the progress curves from the evaluated rates. The directory tree is as follows:

Code
  |
  ├── InsP5[2OH]
  |       |   fitted_exp_data.py
  |       |   minimization_routine.py
  |
  |      
  └── InsP6
          |   fitted_exp_data.py
          |   minimization_routine.py