A great writeup on what calcium imaging measures, what is dF/F0, what is F0 and spike inference #1488
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An excellent recent writeup by Peter Rupprecht. We should add links to this in the docs in multiple places because it comprehensively answers a lot of common questions from uses. Highly recommended reading for newcomers
https://www.scientifica.uk.com/learning-zone/how-to-compute-%CE%B4f-f-from-calcium-imaging-data
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