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22 changes: 22 additions & 0 deletions docs/rnaseq-rmd.rst
Original file line number Diff line number Diff line change
Expand Up @@ -544,6 +544,28 @@ enrichment RMarkdown document.

The output of sessionInfo records the versions of packages used in the analysis.

Errors
------

This section addresses errors encountered during the RNA-Seq downstream analysis.

Error: "Found duplicate names after removing pattern ^contr_[^_]+_"

Cause:
- If no duplicate contrast names exist in your ``results_##`` chunks,
the error can arise from changes to previously cached chunks.
Even if the change is undone and the environment is cleared,
the error may persist. For example, changing ``contr_01``
in ``results_01`` to ``contr_05`` and then reknitting the file
will cause the R environment to contain both ``contr_01`` and
``contr_05`` patterns after that chunk is loaded, leading to
this error.

Solution:
- Clear all cache and R environment objects.
Quit R without saving the workspace, delete the .RData file,
remove the ``rnaseq_cache`` directory, open rnaseq.Rmd and re-knit.

Glossary
--------
.. glossary::
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2 changes: 1 addition & 1 deletion lib/lcdbwf/R/helpers.R
Original file line number Diff line number Diff line change
Expand Up @@ -81,7 +81,7 @@ collect_objects <- function(pattern, fixed=FALSE){
# If there was a wildcard in the pattern there is a risk that the modified
# names are no longer unique
if (length(unique(modified_names)) != length(var_names)){
stop(paste("Found duplicate names after removing pattern", pattern))
stop(paste("Found duplicate names after removing pattern", pattern, "see https://lcdb.github.io/lcdb-wf/rnaseq-rmd.html#Errors for details"))
}
names(obj_list) <- modified_names
return(obj_list)
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