ScanNeo v0.3.4

Fix

• Moved script for preparing hla input to workflow/scripts/genotyping
• Added missing pipe to log file in rule hlatyping_mhcII
• Added wrong input file to filtering of mhcII reads on SE

ScanNeo2 v0.3.3

### Fix

• Fixed parameter in .tests/integration/config_basic/config.yaml (align) to match general config

ScanNeo v0.3.2

Fix

• Removed fixed versions for bwa (0.7.19) and samtools (1.21) to allow installation of the latest versions.
• Updated the VEP wrapper version from "v1.31.1/bio/vep/annotate" to "v5.9.0/bio/vep/annotate".
• Modified functions for file type handling: adjusted indentation in get_preproc_input, updated get_input_filter_reads_mhcII_PE to handle BAM files directly, renamed get_aligned_reads to get_aligned_reads_featurecounts, added new function get_output_hlatyping_mhcII, and updated rule input parameters accordingly.

ScanNeo v0.3.1

Fix

• Changed protocol for HLA alleles reference list to https (rule get_hla_info)
• Fixed path to input files in finalize_mhcII_input.py (which caused error when using paired-end reads)
• Fixed bug in quantification/featurecounts - supported regardless of input type (PE or SE): added wrapper script
• replaced MHC-II binding affinity prediction with IEDB API
• Manual Dockerfile replaces containerized version

ScanNeo2 v0.3.0

Features

• Added sequence similarity filter for MHC-I
  • self-similarity (using kernel similarity)
  • pathogen similarity (BLAST against pathogen-derived epitopes from IEDB)
  • proteome similarity (BLAST against human proteome)
• Prioritization of neoantigens is now done separately for each variant type (speeds up the process)
• Update to recent version of ScanExitron (Thanks to @dolittle007)
  • this version updated to recent version of regtools (v0.5.0) - which is available on Conda
  • Singularity/Docker is not necessary anymore
  • Added option to use strand information in exitron calling
• ScanNeo2 now uses conda environments for all tools (ditched Singularity/Docker)

Fix

• renamed similarity fields for pathogen and protein to more descriptive names

ScanNeo2 v0.2.12

Fix

• Fixed missing alleles in HLA alleles reference list - #34

ScanNeo2 v0.2.11

Fix

• Updated transindel environment to recent samtools version (as --o introduced in samtools >= 1.13 required by transindel)

ScanNeo2 v0.2.10

Fix

• Force combining multiple VCF files in indel detection using mutect2 (e.g., when multiple samples are provided)

ScanNeo2 v0.2.9

Fix

• Splitted rules in HLA typing to ensure better distribution of the workload
• Changed order in HLA typing rules (BAM files are now part of single-end)
  • samtools fastq is only called for BAM files
  • input of filtering directly from preprocessed/raw reads

ScanNeo2 v0.2.8

Fix

• Added threads option to samtools sort calls to speed up the process
• Fixed wrong call to optitype within the wrapper script