Ab Initio Whole Cell Kinetic Model of Bifidobacterium bifidum BGN4 (bbfMA24)

Citation: Arivazhagan, M, Senthilkumar, A, Yeo, KY, Saisudhanbabu, T, Le, MA, Wong, TBS, Lukianto, VR, Ling, MHT. 2025. Ab Initio Whole Cell Kinetic Model of Bifidobacterium bifidum BGN4 (bbfMA24). Acta Scientific Nutritional Health 9(1): 42-45.

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Bifidobacterium bifidum is a common probiotic in human gut and has been shown to be beneficial in many disorders. B. bifidum BGN4 is recognised by US FDA as “generally recognised as safe” for use in infant formulations among other food applications, leading to potential engineered probiotics applications. Mathematical kinetic models provide time-course profile of modelled metabolites, which can be used to guide metabolic engineering approaches. However, there is no kinetic model of B. bifidum to-date. In this study, we present a whole cell simulatable kinetic model of B. bifidum BGN4, bbfMA24, constructed using ab initio approach by identifying enzymes from its published genome. The resulting model consists of 236 metabolites, 68 enzymes with corresponding transcriptions and translations, and 162 enzymatic reactions; which can be a baseline model for incorporating other cellular and growth processes, or as a system to examine cellular resource allocations necessary for engineering.