Metabolite Overproduction Potential of Saccharomyces cerevisiae S288C Explored Using Its Genome Scale Metabolic Model, iMM904.

Citation: Sim, BJH, Wong, KM, Ling, MHT. 2022. Metabolite Overproduction Potential of Saccharomyces cerevisiae S288C Explored Using Its Genome-Scale Metabolic Model, iMM904. EC Microbiology 18(7): 46-51.

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While many genome-scale metabolic models (GSMs) had been constructed with specific purpose, it can be used to explore the potential of the organism for metabolite overproduction. Saccharomyces cerevisiae is a widely used microorganism in biotechnology with many successful applications and a GSM, iMM904, has been constructed for S. cerevisiae S288C. In this study, explore the metabolite overproduction capabilities of S. cerevisiae S288C given single gene knockout using iMM904. Our simulation results suggest that 217 of the 1577 (13.76%) single reaction knockouts potentially leads to metabolite overproductions. This suggests the potential of overproducing native metabolites using only gene knockouts and forms the basis of future validation studies between S. cerevisiae S288C and its corresponding GSM.